Journal article
Lauren Slesur, A. Vilgelm, Rami N. Al-Rohil, A. Richmond
2018
2018
Semantic Scholar
DOI
Cite
Cite
APA
Click to copy
Slesur, L., Vilgelm, A., Al-Rohil, R. N., & Richmond, A. (2018). Abstract B166: Investigating the effect of CDK4/6 and MDM2 inhibition on melanoma.
Chicago/Turabian
Click to copy
Slesur, Lauren, A. Vilgelm, Rami N. Al-Rohil, and A. Richmond. “Abstract B166: Investigating the Effect of CDK4/6 and MDM2 Inhibition on Melanoma” (2018).
MLA
Click to copy
Slesur, Lauren, et al. Abstract B166: Investigating the Effect of CDK4/6 and MDM2 Inhibition on Melanoma. 2018.
BibTeX Click to copy
@article{lauren2018a,
title = {Abstract B166: Investigating the effect of CDK4/6 and MDM2 inhibition on melanoma},
year = {2018},
author = {Slesur, Lauren and Vilgelm, A. and Al-Rohil, Rami N. and Richmond, A.}
}
Abstract
Introduction: The purpose was to investigate how inhibition of MDM2 and CDK4/6 would affect growth of melanoma tumors with deleted CDKN2A in vivo. Methods: B16F0 and WM115 cells were cultured with increasing concentrations of MDM2 inhibitors nutlin-3a, RG7112, or RG7388. Viability was monitored by CellTiter-Blue assay. Induction of p21, MDM2, and BAX expression was monitored by qRT-PCR. C57BL/6J mice (n=40) were injected subcutaneously into both flanks with 50,000 B16F0 cells, a CDKN2A-deficient mouse melanoma cell line. Tumors grew for one week, then mice were randomized into four treatment groups: vehicle (100 µL 0.5% methylcellulose) (n=10), CDK4/6 inhibitor palbociclib 100 mg/kg (n=10), RG7388 150 mg/kg (n=10), and combination palbociclib 100 mg/kg + RG7388 150 mg/kg (n=10). Drugs were given daily via oral gavage. Tumor volumes were measured every 2-3 days, and mice with tumors >15 mm in diameter were euthanized. After 12 days of therapy, tumors were excised and weighed. At the time of euthanasia, mice were bled to evaluate AST and ALT levels. Four tumors per group were randomly selected for HE 2017 Oct 26-30; Philadelphia, PA. Philadelphia (PA): AACR; Mol Cancer Ther 2018;17(1 Suppl):Abstract nr B166.
